Scientific Papers
MycoKey aims to unlock new knowledge and to valorize existing knowledge and data by rapid dissemination to research partners and stakeholders in the chain.
In the MycoKey programme all scientific peer reviewed publications are available through open access. Research data will be deposited in public data repositories for (re)-analysis exploitation and dissemination free of charge.
In this page you can find publications describing the scientific results arising from project’s activities.
TaqMan qPCR for Quantification of Clonostachys rosea Used as a Biological Control Agent Against Fusarium graminearum
In the current study, we developed a quantitative real-time PCR detection method that amends the currently available culture-dependent techniques by using TaqMan chemistry with a highly specific primer and probe set, targeting the actin gene. We established a sensitive assay that detects the biological control agent down to 100 genome copies per reaction, with PCR efficiencies between 90 and 100%. The specificity of the assay was confirmed against a panel of 30 fungal and 3 bacterial species including 12 members of the Fusarium head blight complex and DNA of barley, maize and wheat.
Authors: Alejandro Gimeno1, Elina Sohlberg, Tiina Pakula, Jenni Limnell, Beat Keller, Arja Laitila, Susanne Vogelgsang
Keywords: TaqManq; PCR; Clonostachys rosea; Fusarium graminearum
Published: 16/07/2019
Repository: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020406/
This work has received funding form the the EU’s H2020 research and innovation programme under GA No 678781- MycoKey. CC BY 4.0 license http://creativecommons.org/licenses/by/4.0/
