Scientific Papers

MycoKey aims to unlock new knowledge and to valorize existing knowledge and data by rapid dissemination to research partners and stakeholders in the chain.
In the MycoKey programme all scientific peer reviewed publications are available through open access. Research data will be deposited in public data repositories for (re)-analysis exploitation and dissemination free of charge.
In this page you can find publications describing the scientific results arising from project’s activities, and proceedings from international conferences and workshops.

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Scientific Papers
Variation in Fumonisin and Ochratoxin Production...
Variation in Fumonisin and Ochratoxin Production... Image

This paper shows the high risk due to the contamination of important food crops (cereals, grape and dried fruit) by high genetically different fungal species of Aspergillus genus that produce Ochratoxin A and Fumonisins, which are cancer related metabolites. The studies of the genetics of these Aspergilli are very important for a better prevention in the field.

Authors: Susca Antonia ; Proctor Robert H.; Morelli Massimiliano; Haidukowski Miriam; Gallo Antonia; Logrieco Antonio F; Moretti Antonio
Keywords: fum cluster, ota cluster, fumonisin, ochratoxin, biosynthetic gene cluster, Aspergillus niger, Aspergillus welwitschiae
Published: 9/9/2016
Repository: https://www.zenodo.org/record/161680

 


This work has received funding form the  the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution
(CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators
Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators Image
 This paper reports the possibility that a laccase enzyme produced by a mushroom, Pleurotus pulmonarius, could degrade the carcinogenic mycotoxin aflatoxin B1. This activity can be very useful, since opens the perspective of applying such enzyme in the food and feed supply chains for reducing eventual AFB1 contamination.


Authors: Martina Loi, Francesca Fanelli, Paolo Zucca, Vania C. Liuzzi , Laura Quintieri, Maria T. Cimmarusti, Linda Monaci, Miriam Haidukowski, Antonio F. Logrieco, Enrico Sanjust, Giuseppina Mulè.
Keywords: laccase; Pleurotus; mycotoxins; aflatoxin B1; aflatoxin M1; biodegradation; redox mediators
Published: 23/8/2016

This work has received funding form the the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution
(CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Products
Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Products Image

An unprecedented, environmentally friendly, and faster method for the determination of Ochratoxin A (OTA) (a mycotoxin produced by several species of Aspergillus and Penicillium and largely widespread in nature, in wheat and derived products) has, for the first time, been set up and validated using choline chloride (ChCl)-based deep eutectic solvents (DESs) (e.g., ChCl/glycerol (1:2) and ChCl/ urea (1:2) up to 40% (w/w) water) as privileged, green, and biodegradable extraction solvents. This also reduces worker exposure to toxic chemicals. Results are comparable to those obtained using conventional, hazardous and volatile organic solvents (VOCs) typical of the standard and official methods.

Authors: Luca Piemontese, Filippo Maria Perna, Antonio Logrieco, Vito Capriati, Michele Solfrizzo.
Repository: https://www.zenodo.org/record/252327
Keywords: deep eutectic solvents; Ochratoxin A; food chemistry; analytical method; green solvents
Published: 12/01/2017

This work has received funding form the the EU’s H2020 research and innovation programme under GA No 678781- MycoKey. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Enniatin and Beauvericin Biosynthesis in Fusarium Species: Production Profiles and Structural Determinant Prediction
Enniatin and Beauvericin Biosynthesis in Fusarium Species: Production Profiles and Structural Determinant Prediction Image

Members of the fungal genus Fusarium can produce numerous secondary metabolites, including the nonribosomal mycotoxins beauvericin (BEA) and enniatins (ENNs). Both mycotoxins are synthesized by the multifunctional enzyme enniatin synthetase (ESYN1) that contains both peptide synthetase and S-adenosyl-l-methionine-dependent N-methyltransferase activities. Several Fusarium species can produce ENNs, BEA or both, but the mechanism(s) enabling these differential metabolic profiles is unknown. In this study, we analyzed the primary structure of ESYN1 by sequencing esyn1 transcripts from different Fusarium species.

Authors: Vania C. Liuzzi, Valentina Mirabelli, Maria Teresa Cimmarusti, Miriam Haidukowski, John F. Leslie, Antonio F. Logrieco, Rocco Caliandro,Francesca Fanelli, Giuseppina Mulè.
Repository: https://www.zenodo.org/record/264018
Keywords: enniatin; beauvericin; ESYN1; mycotoxins; Fusarium; homology modelling; multivariate analysis; backbone angles
Published: 25/01/2017

This work has received funding form the the EU’s H2020 research and innovation programme under GA No 678781- MycoKey. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Scientific Papers
Variation in Fumonisin and Ochratoxin Production... Image
Variation in Fumonisin and Ochratoxin Production...
This paper shows the high risk due to the contamination of important food crops (cereals, grape and dried fruit) by high genetically different fungal species of Aspergillus genus that produce Ochratoxin A and... Read More
Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators Image
Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators
 This paper reports the possibility that a laccase enzyme produced by a mushroom, Pleurotus pulmonarius, could degrade the carcinogenic mycotoxin aflatoxin B1. This activity can... Read More
Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Products Image
Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Products
An unprecedented, environmentally friendly, and faster method for the determination of Ochratoxin A (OTA) (a mycotoxin produced by several species of Aspergillus and Penicillium and largely widespread in nature, in wheat an... Read More
Enniatin and Beauvericin Biosynthesis in Fusarium Species: Production Profiles and Structural Determinant Prediction Image
Enniatin and Beauvericin Biosynthesis in Fusarium Species: Production Profiles and Structural Determinant Prediction
Members of the fungal genus Fusarium can produce numerous secondary metabolites, including the nonribosomal mycotoxins beauvericin (BEA) and enniatins (ENNs). Both mycotoxins are synthesized by the multifunctional enzyme enniatin ... Read More