Scientific Papers

MycoKey aims to unlock new knowledge and to valorize existing knowledge and data by rapid dissemination to research partners and stakeholders in the chain.
In the MycoKey programme all scientific peer reviewed publications are available through open access. Research data will be deposited in public data repositories for (re)-analysis exploitation and dissemination free of charge.
In this page you can find publications describing the scientific results arising from project’s activities, and proceedings from international conferences and workshops.

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Scientific Papers
Variation in Fumonisin and Ochratoxin Production Associated with Differences in Biosynthetic Gene Content in Aspergillus niger and A. welwitschiae Isolates from Multiple Crop and Geographic Origins
Variation in Fumonisin and Ochratoxin Production Associated with Differences in Biosynthetic Gene Content in Aspergillus niger and A. welwitschiae Isolates from Multiple Crop and Geographic Origins Image

This paper shows the high risk due to the contamination of important food crops (cereals, grape and dried fruit) by high genetically different fungal species of Aspergillus genus that produce Ochratoxin A and Fumonisins, which are cancer related metabolites. The studies of the genetics of these Aspergilli are very important for a better prevention in the field.

Authors: Susca Antonia; Proctor Robert H; Morelli Massimiliano; Haidukowski Miriam; Gallo Antonia; Logrieco Antonio F; Moretti Antonio
Keywords: fum cluster, ota cluster, fumonisin, ochratoxin, biosynthetic gene cluster, Aspergillus niger, Aspergillus welwitschiae
Published: 9/9/2016
Repository: https://www.zenodo.org/record/161680


This work has received funding form the  the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
CC-BY license http://creativecommons.org/licenses/by/4.0/

 

Mycotoxin Biotransformation by Native and Commercial Enzymes: Present and Future Perspectives
Mycotoxin Biotransformation by Native and Commercial Enzymes: Present and Future Perspectives Image

This review focuses on the biotransformation of mycotoxins performed with purified enzymes isolated from bacteria, fungi and plants, whose activity was validated in in vitro and in vivo assays, including patented ones and commercial preparations. Furthermore, we will present some applications for detoxifying enzymes in food, feed, biogas and biofuel industries, describing their limitation and potentialities.

Authors: Martina Loi, Francesca Fanelli, Vania C. Liuzzi, Antonio F. Logrieco, Giuseppina Mulè
Repository: https://zenodo.org/record/466207
Keywords: mycotoxins; biotransformation; degradation; enzymes; application
Published: 24/03/2017

This work has received funding form the  the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
CC-BY license http://creativecommons.org/licenses/by/4.0/

 

 

Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators
Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators Image
 This paper reports the possibility that a laccase enzyme produced by a mushroom, Pleurotus pulmonarius, could degrade the carcinogenic mycotoxin aflatoxin B1. This activity can be very useful, since opens the perspective of applying such enzyme in the food and feed supply chains for reducing eventual AFB1 contamination.

Authors: Martina Loi, Francesca Fanelli, Paolo Zucca, Vania C. Liuzzi , Laura Quintieri, Maria T. Cimmarusti, Linda Monaci, Miriam Haidukowski, Antonio F. Logrieco, Enrico Sanjust, Giuseppina Mulè.
Keywords: laccase; Pleurotus; mycotoxins; aflatoxin B1; aflatoxin M1; biodegradation; redox mediators
Published: 23/8/2016
Repository: https://www.zenodo.org/record/161671


This work has received funding form the  the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
CC-BY license http://creativecommons.org/licenses/by/4.0/

 

Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Products
Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Products Image

An unprecedented, environmentally friendly, and faster method for the determination of Ochratoxin A (OTA)  has for the first time, been set up and validated using choline chloride (ChCl)-based deep eutectic solvents (DESs) (e.g., ChCl/glycerol (1:2) and ChCl/ urea (1:2) up to 40% (w/w) water) as privileged, green, and biodegradable extraction solvents.

Authors: Luca Piemontese, Filippo Maria Perna, Antonio Logrieco, Vito Capriati, Michele Solfrizzo.
Repository: https://www.zenodo.org/record/252327
Keywords: deep eutectic solvents; Ochratoxin A; food chemistry; analytical method; green solvents
Published: 12/01/2017

This work has received funding form the  the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
CC-BY license http://creativecommons.org/licenses/by/4.0/

 

Decontamination of Fumonisin B1 in maize grain by Pleurotus eryngii and antioxidant enzymes
Decontamination of Fumonisin B1 in maize grain by Pleurotus eryngii and antioxidant enzymes Image

This paper investigates the potential role of P. eryngii in the decontamination of FB1 in maize. Selected antioxidant enzymes, (soluble peroxidase (POD), catalase (CAT) and ascorbate peroxidase), primarily involved in control of cell hydrogen peroxide levels, and lignin degradation, were analyzed, to evaluate their contributions to the molecular mechanisms of FB1 by P. eryngii.

Authors: Miriam Haidukowski; Giuseppe Cozzi; Nunzio Dipierro; Simona L. Bavaro; Antonio F. Logrieco; Costantino Paciolla
Keywords: fungal strains, fumonisin B1, hydrogen peroxide, decontamination, mycotoxins.
Published: 10/05/2017
Repository: https://www.zenodo.org/record/574663


This work has received funding form the  the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
CC-BY license http://creativecommons.org/licenses/by/4.0/

Enniatin and Beauvericin Biosynthesis in Fusarium Species: Production Profiles and Structural Determinant Prediction
Enniatin and Beauvericin Biosynthesis in Fusarium Species: Production Profiles and Structural Determinant Prediction Image

In this study, we analyzed the primary structure of ESYN1 by sequencing esyn1 transcripts from different Fusarium species. Members of the fungal genus Fusarium can produce numerous secondary metabolites, including the nonribosomal mycotoxins beauvericin (BEA) and enniatins (ENNs). Both mycotoxins are synthesized by the multifunctional enzyme enniatin synthetase (ESYN1) that contains both peptide synthetase and S-adenosyl-l-methionine-dependent N-methyltransferase activities.

Authors: Vania C. Liuzzi, Valentina Mirabelli, Maria Teresa Cimmarusti, Miriam Haidukowski, John F. Leslie, Antonio F. Logrieco, Rocco Caliandro,Francesca Fanelli, Giuseppina Mulè.
Repository: https://www.zenodo.org/record/264018
Keywords: enniatin; beauvericin; ESYN1; mycotoxins; Fusarium; homology modelling; multivariate analysis; backbone angles
Published: 25/01/2017

This work has received funding form the  the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
CC-BY license http://creativecommons.org/licenses/by/4.0/

 

Microbial Detoxification of Deoxynivalenol (DON), Assessed via a Lemna minor L. Bioassay, through Biotransformation to 3-epi-DON and 3-epi-DOM-1
Microbial Detoxification of Deoxynivalenol (DON), Assessed via a Lemna minor L. Bioassay, through Biotransformation to 3-epi-DON and 3-epi-DOM-1 Image

Mycotoxins are toxic metabolites produced by fungi. To mitigate mycotoxins in food or feed, biotransformation is an emerging technology in which microorganisms degrade toxins into non-toxic metabolites. To monitor deoxynivalenol (DON) biotransformation, analytical tools such as ELISA and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) are typically used. However, these techniques do not give a decisive answer about the remaining toxicity of possible biotransformation products. Hence, a bioassay using Lemna minor L. was developed.

Authors: Vanhoutte I; De Mets L; De Boevre M; Uka V; Di Mavungu JD; De Saeger S; De Gelder L; Audenaert K.
Keywords: deoxynivalenol (DON); Lemna minor; bioassay; biotransformation; detoxification; 3-epi-DON; 3-epi-de-epoxy-DON (3-epi-DOM-1)
Published: 13/02/2017
Repository: https://biblio.ugent.be/publication/8512245


This work has received funding form the  the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
CC-BY license http://creativecommons.org/licenses/by/4.0/

Scientific Papers
Variation in Fumonisin and Ochratoxin Production Associated with Differences in Biosynthetic Gene Content in Aspergillus niger and A. welwitschiae Isolates from Multiple Crop and Geographic Origins Image
Variation in Fumonisin and Ochratoxin Production Associated with Differences in Biosynthetic Gene Content in Aspergillus niger and A. welwitschiae Isolates from Multiple Crop and Geographic Origins
This paper shows the high risk due to the contamination of important food crops (cereals, grape and dried fruit) by high genetically different fungal species of Aspergillus genus that produce Ochratoxin A and... Read More
Mycotoxin Biotransformation by Native and Commercial Enzymes: Present and Future Perspectives Image
Mycotoxin Biotransformation by Native and Commercial Enzymes: Present and Future Perspectives
This review focuses on the biotransformation of mycotoxins performed with purified enzymes isolated from bacteria, fungi and plants, whose activity was validated in in vitro and in vivo assays, including patented ones and commercial prep... Read More
Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators Image
Aflatoxin B1 and M1 Degradation by Lac2 from Pleurotus pulmonarius and Redox Mediators
 This paper reports the possibility that a laccase enzyme produced by a mushroom, Pleurotus pulmonarius, could degrade the carcinogenic mycotoxin aflatoxin B1. This activity can... Read More
Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Products Image
Deep Eutectic Solvents as Novel and Effective Extraction Media for Quantitative Determination of Ochratoxin A in Wheat and Derived Products
An unprecedented, environmentally friendly, and faster method for the determination of Ochratoxin A (OTA)  has for the first time, been set up and validated using choline chloride (ChCl)-based deep eutectic solvents (DESs) (e.g., ChCl/g... Read More
Decontamination of Fumonisin B1 in maize grain by Pleurotus eryngii and antioxidant enzymes Image
Decontamination of Fumonisin B1 in maize grain by Pleurotus eryngii and antioxidant enzymes
This paper investigates the potential role of P. eryngii in the decontamination of FB1 in maize. Selected antioxidant enzymes, (soluble peroxidase (POD), catalase (CAT) and ascorbate peroxidase), primarily involved in control of cell hyd... Read More
Enniatin and Beauvericin Biosynthesis in Fusarium Species: Production Profiles and Structural Determinant Prediction Image
Enniatin and Beauvericin Biosynthesis in Fusarium Species: Production Profiles and Structural Determinant Prediction
In this study, we analyzed the primary structure of ESYN1 by sequencing esyn1 transcripts from different Fusarium species. Members of the fungal genus Fusarium can produce numerous secondary metabolites, including th... Read More
Microbial Detoxification of Deoxynivalenol (DON), Assessed via a Lemna minor L. Bioassay, through Biotransformation to 3-epi-DON and 3-epi-DOM-1 Image
Microbial Detoxification of Deoxynivalenol (DON), Assessed via a Lemna minor L. Bioassay, through Biotransformation to 3-epi-DON and 3-epi-DOM-1
Mycotoxins are toxic metabolites produced by fungi. To mitigate mycotoxins in food or feed, biotransformation is an emerging technology in which microorganisms degrade toxins into non-toxic metabolites. To monitor deoxynivalenol (DON) bi... Read More