Scientific Papers
MycoKey aims to unlock new knowledge and to valorize existing knowledge and data by rapid dissemination to research partners and stakeholders in the chain.
In the MycoKey programme all scientific peer reviewed publications are available through open access. Research data will be deposited in public data repositories for (re)-analysis exploitation and dissemination free of charge.
In this page you can find publications describing the scientific results arising from project’s activities.
A loop-mediated isothermal amplification (LAMP) assay for rapid detection of fumonisin producing Aspergillus species
In this study, we developed a LAMP assay, based on the detection of fum10, for a rapid and specific molecular detection of FB2-producing A. niger and A. welwistchiae, potentially useful to perform monitoring directly “on site” in maize chain. Results showed that very low amounts of conidia are suitable to detect the presence of the target gene, thus providing information about the presence of FB2-producing Aspergillus species and the possible upcoming fumonisins contamination in maize. The assay was combined with a suitable protocol for “in field” crude DNA extraction and a colorimetric method for easy naked-eye evaluationof results, offering a reliable and user-friendly tool to support effective reduction strategies of mycotoxin contamination in crop management programs.
Authors: Massimo Ferrara, Antonio F. Logrieco, Antonio Moretti, Antonia Susca
Keywords: Aspergillus niger, Aspergillus welwitschiae, Rapid molecular tool, Fumonisin, Maize
Published: 28/02/2020
Repository: https://zenodo.org/record/3752726#.XpboW8gzbIU
This work has received funding form the the EU’s H2020 research and innovation programme under GA No 678781- MycoKey. CC BY 4.0 license http://creativecommons.org/licenses/by/4.0/
