Scientific Papers
MycoKey aims to unlock new knowledge and to valorize existing knowledge and data by rapid dissemination to research partners and stakeholders in the chain.
In the MycoKey programme all scientific peer reviewed publications are available through open access. Research data will be deposited in public data repositories for (re)-analysis exploitation and dissemination free of charge.
In this page you can find publications describing the scientific results arising from project’s activities.
A CRISPR- Cas9 System for Genome Editing of Fusarium proliferatum
In this study, we used a Type II CRISPR-Cas9 system to inactivate a key gene in the fumonisin biosynthetic cluster in F. proliferatum. Our working hypotheses were: (i) in-vitro-assembled RNPs could be used with a donor DNA repair template for direct co-transformation of F. proliferatum, and (ii) the FUM1 gene was essential for the synthesis of fumonisin. This work advances the field by providing a new, simple tool for making knockout mutants in members of the F. fujikuroi species complex, and by confirming the essential role of FUM1 in fumonisin biosynthesis in F. proliferatum.
Authors: Ferrara, M., Haidukowski, M., Logrieco, A.F., Leslie, J. F., Mulè, G
Keywords: CRISPR- Cas9; Genome Editing; Fusarium
Published: 27/12/2019
Repository: https://www.ncbi.nlm.nih.gov/pubmed/31882627?dopt=Abstract
This work has received funding form the the EU’s H2020 research and innovation programme under GA No 678781- MycoKey.
CC-BY license http://creativecommons.org/
